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Image Search Results
Journal: Molecular Plant Pathology
Article Title: Helper component‐proteinase (HC‐Pro) protein of Papaya ringspot virus interacts with papaya calreticulin
doi: 10.1111/j.1364-3703.2009.00606.x
Figure Lengend Snippet: In vitro protein‐binding assay of Papaya ringspot virus helper component‐proteinase (PRSV HC‐Pro) and Carica papaya calreticulin (PaCRT). In vitro translated biotinylated PaCRT was incubated with purified glutathione S‐transferase (GST) or GST‐PRSV HC‐Pro, which was immobilized on MagneGST particles. After incubation, the proteins interacting with GST or GST‐PRSV HC‐Pro were bound to the MagneGST particles. The eluted GST‐bound proteins (lane 4) or GST‐PRSV HC‐Pro‐bound proteins (lane 5) were then analysed by 10% sodium dodecylsulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and probed with streptavidin‐alkaline phosphatase (streptavidin‐AP) (A) or anti‐GST antibody (B) (GST, 26.0 kDa; GST‐PRSV HC‐Pro, 78.0 kDa; biotinylated PaCRT, 48.2 kDa). The purified GST (lane 1), purified GST‐PRSV HC‐Pro (lane 2) and in vitro translated biotinylated PaCRT (lane 3) were used as controls, respectively. The prestained dual colour protein size marker (Tiangen) is indicated on the left of the panel.
Article Snippet: The
Techniques: In Vitro, Protein Binding, Incubation, Purification, Polyacrylamide Gel Electrophoresis, SDS Page, Marker